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Alomone Labs vendor apc cy7 cd45 ox 1 bd pe cy7 cd11b c ox 42 bd fitc p2y12 n
(A): Representative samples from both sham and CCI groups are shown. The single cell population was identified based on SSC and FSC. Live cells were identified as negative for the 7-AAD. Microglia were identified using a two-step method. First, <t>CD11b/c+</t> cells <t>(PE-Cy7)</t> were selected to identify all myeloid cells. The CD11b/c+ cells were then gated on <t>P2Y12</t> <t>(FITC)</t> and <t>CD45</t> <t>(APC-Cy7).</t> Microglia were identified as triple positive cells. SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells.
Vendor Apc Cy7 Cd45 Ox 1 Bd Pe Cy7 Cd11b C Ox 42 Bd Fitc P2y12 N, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A): Representative samples from both sham and CCI groups are shown. The single cell population was identified based on SSC and FSC. Live cells were identified as negative for the 7-AAD. Microglia were identified using a two-step method. First, CD11b/c+ cells (PE-Cy7) were selected to identify all myeloid cells. The CD11b/c+ cells were then gated on P2Y12 (FITC) and CD45 (APC-Cy7). Microglia were identified as triple positive cells. SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells.

Journal: Stem cells (Dayton, Ohio)

Article Title: Combination therapy with Treg and MSC enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy

doi: 10.1002/stem.3320

Figure Lengend Snippet: (A): Representative samples from both sham and CCI groups are shown. The single cell population was identified based on SSC and FSC. Live cells were identified as negative for the 7-AAD. Microglia were identified using a two-step method. First, CD11b/c+ cells (PE-Cy7) were selected to identify all myeloid cells. The CD11b/c+ cells were then gated on P2Y12 (FITC) and CD45 (APC-Cy7). Microglia were identified as triple positive cells. SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells.

Article Snippet: SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Fluorochrome Antibody Clone Vendor APC-Cy7 CD45 OX-1 BD PE-Cy7 CD11b/c OX-42 BD FITC P2Y12 n/a Alomone Labs PE CD32 D34–485 BD Alexa Fluor 647 RT1B OX-6 BD PerCP Cy5.5 7-AAD n/a BD Open in a separate window Multicolor Flow Cytometry Microglia/Myeloid Cell Panel Microglia gating strategy Conventional flow cytometry analyses were performed with FlowJo vr10.6.1.

Techniques: Flow Cytometry

Multicolor Flow Cytometry Microglia/Myeloid Cell Panel

Journal: Stem cells (Dayton, Ohio)

Article Title: Combination therapy with Treg and MSC enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy

doi: 10.1002/stem.3320

Figure Lengend Snippet: Multicolor Flow Cytometry Microglia/Myeloid Cell Panel

Article Snippet: SSC, side scatter; FSC, forward scatter. (B): The multiple color flow cytometry panel used to identify and immunophenotype microglia and peripheral myeloid cells. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Fluorochrome Antibody Clone Vendor APC-Cy7 CD45 OX-1 BD PE-Cy7 CD11b/c OX-42 BD FITC P2Y12 n/a Alomone Labs PE CD32 D34–485 BD Alexa Fluor 647 RT1B OX-6 BD PerCP Cy5.5 7-AAD n/a BD Open in a separate window Multicolor Flow Cytometry Microglia/Myeloid Cell Panel Microglia gating strategy Conventional flow cytometry analyses were performed with FlowJo vr10.6.1.

Techniques: Flow Cytometry